| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | North Central | A | Milburn | Davis | Smith | Goudeau | 5:12.58 | Verified |
| 2 | Grand Lake | A | Reeves | Cox | Sonnier | Bergeron | 4:50.39 | Verified |
| 3 | Westminster Christian Academy | A | Wright | Amy | Davis | Lalonde | 4:46.67 | Verified |
| 4 | Merryville | A | Hooper | Lewis | Johnson | Mahfouz | 4:26.81 | Verified |
| 5 | Highland Baptist Christian Schoo | A | Sanchez | Abshire | Champagne | Champagne | 4:42.17 | Verified |
| 6 | Basile | A | David | Lejeune | Manuel | Alvarez | 4:47.59 | Verified |
| 7 | Vermilion Catholic | A | Guarino | Griffin | Elena Desormeaux | Meaux | 5:09.72 | Verified |
| 8 | East Beauregard | A | Gallien | Sweat | Dickens | Eggers | 5:17.57 | Verified |