| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 2 | DAVIS, DESTINY | North Central | 53.97 | Verified |
| 3 | HICKMAN, KAILEE | Merryville | 52.16 | Verified |
| 4 | MAHFOUZ, MADISEN | Merryville | 49.03 | Verified |
| 5 | CALAIS, AVA CLAIRE | Central Catholic | 50.35 | Verified |
| 6 | DAVIS, EMILY | Covenant Christian | 52.68 | Verified |
| 7 | MUDD, MARCIE | Grand Lake | 55.01 | Verified |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 2 | GALLIEN, HILLARY | East Beauregard | 1:03.89 | Verified |
| 3 | BOBB, ALYIAH | Westminster Christian Academy | 57.31 | Verified |
| 4 | ABSHIRE, DUSTI | Highland Baptist Christian Schoo | 55.46 | Verified |
| 5 | BREAUX, FAITH | Highland Baptist Christian Schoo | 56.27 | Verified |
| 6 | CLOUD, CARLY | Sacred Heart Ville Platte | 58.24 | Verified |